2026
12. Schulz-Mirbach, H., Rainaldi, V., Bohra, N., Suzuki, K., Danet, T., Kasim, H., Satanowski, A., He, H., Rossini, E., Lee, S. H., Klose, M., Kahnt, J., Glatter, T., Claus, P., Paczia, N., Dronsella, B., Luo, S., Claassens, N., Erb, T. J. (2026) Combining evolution and machine learning-guided pathway optimization to engineer a novel methylsuccinate module for synthetic C1 metabolism in vivo. bioRxiv 2026.01.16.699985; https://doi.org/10.64898/2026.01.16.699985
2025
11. Gasparrini, W., Lee, S. H., & Woolston, B. M. (2025) Pathways to sustainability: a quantitative comparison of aerobic and anaerobic C1 bioconversion routes. Current Opinion in Biotechnology, 93, 103310. https://doi.org/10.1016/j.copbio.2025.103310
10. Lee, S. H., Cirino, P. C. & Gonzalez, R. (2025) Metabolic Engineering of Escherichia coli for the utilization of methylsuccinate, the product of methane activation via fumarate addition. Bioresource Technology, 416, 131700. https://doi.org/10.1016/j.biortech.2024.131700
2024
9. Kim, Y., Lee, S. H., Gade, P., Nattermann, M., Maltseva, N., Endres, M., Chen, J., Wichmann, P., Hu, Y., Marchal, D. G., Yoshikuni, Y., Erb, T., Gonzalez, R., Michalska, K., & Joachimiak, A. (2024) Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family. Communications Chemistry, 7, 160. https://doi.org/10.1038/s42004-024-01242-y
8. Lee, S. H.†, Hu, Y.†, Chou, A., Chen, J., & Gonzalez, R. (2024) Metabolic flux optimization of iterative pathways through orthogonal gene expression control: application to the β-oxidation reversal. Metabolic Engineering, 82, 262-273. († equal contribution) https://doi.org/10.1016/j.ymben.2024.02.007
2023
7. Lee, S. H., Chou, A., Nattermann, M., Zhu, F., Clomburg, J., Paczia, N., Erb, T., & Gonzalez, R. (2023) Identification of 2-hydroxyacyl-CoA synthases with high acyloin condensation activity for orthogonal one-carbon bioconversion. ACS Catalysis, 13, 18, 12007-12020. https://doi.org/10.1021/acscatal.3c02373
6. Nattermann, M., Wenk, S., Pfister, P., He, H., Lee, S. H., Szymanski, W., Guntermann, N., Zhu, F., Nickel, L., Wallner, C., Zarzycki, J., Paczia, N., Gaißert, N., Franciò, G., Leitner, W., Gonzalez, R., & Erb, T. (2023) Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo. Nature Communications, 14(1), 1-14. https://doi.org/10.1038/s41467-023-38072-w
2022
5. Vögeli, B., Schulz, L., Garg, S., Tarasava, K., Clomburg, J., Lee, S. H., Gonnot, A., Moully, E., Kimmel, B., Mrksich, M., Karim, A. S., Gonzalez, R., Köpke, M., & Jewett, M. C. (2022) Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria. Nature Communications, 13, 3058. https://doi.org/10.1038/s41467-022-30571-6.
4. Tarasava, K.†, Lee, S. H.†, Chen, J., Köpke, M., Jewett, M. C., & Gonzalez, R. (2022) Reverse β-oxidation pathways for efficient chemical production. Journal of Industrial Microbiology and Biotechnology, 49(2), kuac003. († equal contribution) https://doi.org/10.1093/jimb/kuac003
3. Wang, Y., Nguyen, N., Lee, S. H., Wang, Q., May, J. A., Gonzalez, R., & Cirino, P. C. (2022) Engineering Escherichia coli for anaerobic alkane activation: Biosynthesis of (1-methylalkyl)succinates. Biotechnology and Bioengineering, 119(1), 315-320. https://doi.org/10.1002/bit.27956
2021
2. Chou, A.†, Lee, S. H.†, Zhu, F.†, Clomburg, J., & Gonzalez, R. (2021) An orthogonal metabolic framework for one-carbon utilization. Nature Metabolism, 3, 1385–1399. († equal contribution) https://doi.org/10.1038/s42255-021-00453-0
1. Nattermann, M., Burgener, S., Pfister, P., Chou, A., Schulz, L., Lee, S. H., Paczia, N., Zarzycki, J., Gonzalez, R., & Erb, T. (2021) Engineering a highly efficient carboligase for synthetic one-carbon metabolism. ACS Catalysis, 11(9), 5396–5404. https://doi.org/10.1021/acscatal.1c01237